LAPORAN KEGIATAN MAGANG MAHASISWA
METODE DETEKSI VIRUS AVIAN INFLUENZA SECARA MOLEKULER DENGAN REVERSE TRANSCRIPTION-POLYMERASE
CHAIN REACTION (RT-PCR) DI BALAI BESAR VETERINER WATES
Laporan ini disusun untuk
memenuhi persyaratan dalam melaksanakan Kegiatan Magang Mahasiswa (KMM)
Disusun oleh:
name
NIM
JURUSAN BIOLOGI
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS SEBELAS MARET
SURAKARTA
2013
LAPORAN KEGIATAN
MAGANG MAHASISWA
METODE DETEKSI VIRUS AVIAN INFLUENZA SECARA MOLEKULER DENGAN REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION (RT-PCR) DI BALAI BESAR VETERINER WATES
name
NIM
Dinyatakan
Sah dan Lengkap
Pada Tanggal Desember 2013
|
Pembimbing Lapangan
name
NIP.
Mengetahui
A.n. Pimpinan Balai Besar Veteriner Wates
name
NIP.
|
Pembimbing
KMM/Magang
name
NIP.
Ketua Jurusan
name
NIP.
|
KATA PENGANTAR
Puji dan syukur Penulis
panjatkan kepada Allah SWT atas segala karunia-Nya sehingga Lapoaran Kegiatan Magang Mahasiswa (KMM) ini berhasil diselesaikan. Tema yang dipilih dalam KMM
yang dilaksanakan pada 19 Juli -19 Agustus 2013 ini
adalah Metode Deteksi Virus Avian Influenza Secara Molekuler,
dengan judul Metode Deteksi Virus Avian Influenza Secara Molekuler Dengan Reverse
Transcription-Polymerase Chain Reaction (RT-PCR) Di Balai Besar Veteriner
Wates. Laporan Kegiatan
Magang Mahasiswa (KMM) berjudul Metode Deteksi Virus Avian Influenza Secara Molekuler Dengan Reverse
Transcription-Polymerase Chain Reaction (RT-PCR) Di Balai Besar Veteriner
Wates terdiri dari Metode RT-PCR, Metode Real Time Reverse
Transcription-Polymerase Chain Reaction, Pengembangan
teknik dan metode diagnosa
penyakit di Laboratorium Bioteknologi Balai
Besar Veteriner Wates.
Materi ini disajikan dengan
sistematis agar dapat dengan mudah dipahami
oleh para pembaca khususnya mahasiswa sehingga akan memperluas wawasan dan pengetahuan mahasiswa secara
teknis tentang teknik RT-PCR
dalam
mendeteksi virus Avian Influenza. Diharapkan dengan memperluas wawasan dan pengetahuan mahasiswa maka akan diiringi juga peningkatan keterampilan mahasiswa di
bidang deteksi
virus Avian Influenza
dengan RT-PCR, sehingga akan berdampak pada meningkatnya kesejahteraan
masyarakat luas pada umumnya.
Surakrta, 04 Desember 2013
name
DAFTAR
ISI
|
|
Latar
Belakang............................................................................................................. 1
Perumusan
Masalah...................................................................................................... 4
Tujuan
KMM................................................................................................................ 5
Manfaat
KMM............................................................................................................. 5
BAB
II. PROFIL INSTITUSI MITRA
Sejarah
Pendirian.......................................................................................................... 6
Visi
dan Misi................................................................................................................ 7
Tugas
dan Fungsi.......................................................................................................... 8
Struktur
Kepengurusan Balai Besar Veteriner Wates.................................................. 10
Pelayanan
di Balai Besar Veteriner Wates................................................................... 11
BAB
III. TINJAUAN PUSTAKA
Morfologi
virus Avian Influenza................................................................................... 14
Sifat
– sifat virus Avian Influenza................................................................................. 16
Penyebaran
virus Avian Influenza................................................................................. 16
Diagnosa Avian Influenza dengan Metode Molekuler.................................................. 16
BAB
IV. PROGRAM KERJA
Waktu dan Tempat....................................................................................................... 19
Alat
dan Bahan............................................................................................................. 19
Cara
Kerja..................................................................................................................... 20
BAB
V. HASIL DAN PEMBAHASAN
Metode
Reverse
Transcription-Polymerase Chain Reaction (RT-PCR)...................... 25
Metode
Real Time Reverse Transcription-Polymerase Chain Reaction...................... 28
Pengembangan
teknik dan metode diagnosa
penyakit di Laboratorium Bioteknologi Balai
Besar Veteriner Wates............................................................................................................................ 31
BAB
VI. PENUTUP
KESIMPULAN........................................................................................................... 33
DAFTAR PUSTAKA.................................................................................................. 34
UCAPAN TERIMAKASIH........................................................................................ 36
DAFTAR PUSTAKA
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